The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products and solutions were being digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A point mutation was released by the https://nielst711lwg4.activosblog.com/profile