Pour the mixture of lysate and Ni‐NTA agarose into the column, and allow it to empty possibly by gravity stream or by applying a vacuum to the bottom with the column. Skip to primary articles Thank you for visiting mother nature.com. You might be utilizing a browser version with limited https://www.facebook.com/NewTechFind/posts/pfbid0C5fmSXJrapHFYG52mcPLSN2nTbXxqHYvipy9fSmo2CxcnbiVbpEedVhmhkpwRBjyl